Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Substrate specificity of choline kinase.

G L Clary, C F Tsai, R W Guynn

Archives of Biochemistry and Biophysics

|April 1, 1987

Summary

Brewer's yeast choline kinase exhibits strict substrate requirements, preferring choline analogs with specific structural features. This enzyme specificity offers insights for designing targeted cholinergic probes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of fermented soybean meal with Bacillus velezensis, Lactobacillus spp. or their combination on broiler performance, gut antioxidant activity and microflora.

Animal bioscience·2022

Same author

First Report of Cowpea mild mottle virus in Cowpea and French Bean in Taiwan.

Plant disease·2019

Same author

Syncope caused by complete heart block and ventricular arrhythmia as early manifestation of systemic lupus erythematosus in a pregnant patient: a case report.

Lupus·2018

Same author

Survey of total mercury in foods from Taiwan containing red mould rice (Monascus) using a direct mercury analyser (DMA).

Food additives & contaminants. Part B, Surveillance·2014

Same author

Differences in brain metabolism associated with agitation and depression in Alzheimer's disease.

East Asian archives of psychiatry : official journal of the Hong Kong College of Psychiatrists = Dong Ya jing shen ke xue zhi : Xianggang jing shen ke yi xue yuan qi kan·2013

Same author

Understanding nanoparticle self-assembly for a strong improvement in functionality in thin film nanocomposites.

Nanotechnology·2010

Area of Science:

Biochemistry
Enzymology

Background:

Choline kinase (ATP:choline phosphotransferase, EC 2.7.1.32) is crucial for phosphorylating choline.
Understanding its substrate specificity is key to elucidating its biological role and potential therapeutic applications.

Purpose of the Study:

To investigate the substrate specificity of choline kinase from brewer's yeast.
To identify structural requirements for choline analogs to act as substrates for this enzyme.

Main Methods:

Enzyme assays were performed using various choline analogs.
Kinetic parameters (Vmax, Km) were determined for active analogs.
Structure-activity relationships were analyzed based on analog modifications.

Main Results:

Brewer's yeast choline kinase demonstrated stringent substrate specificity, unlike many other choline-using systems.
Short alkyl substitutions on the quaternary nitrogen were tolerated, but longer chains and aromatic derivatives were not.
Modifications to the hydroxyethyl side chain, particularly on the beta carbon, severely reduced or eliminated substrate activity.
Single substituents on the alpha carbon were moderately tolerated.

Conclusions:

The enzyme likely interacts with the substrate at four key points: the hydroxyalkyl side chain and three sites on the quaternary nitrogen.
One quaternary nitrogen interaction site appears more sterically hindered.
The findings provide valuable data for designing specific cholinergic probes targeting enzyme or metabolic functions.

Related Experiment Videos