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Long noncoding RNA OPA-interacting protein 5 antisense transcript 1 upregulated SMAD3 expression to contribute to

Xing Chen1, Dongsheng Xiong2, Huichun Yang1

  • 1Department of Obstetrics and Gynecology, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai, Zhejiang, China.

Journal of Cellular Physiology
|October 21, 2018
PubMed
Abstract

Insights

Long noncoding RNA OIP5-AS1 promotes cervical cancer progression by increasing SMAD3 expression. It achieves this by sponging miR-143-3p, ultimately enhancing cell migration and invasion.

Area of Science:

  • Molecular Oncology
  • Cancer Biology

Background:

  • SMAD3 plays a crucial role in the biological functions of various tumors.
  • Understanding the interplay of SMAD3, long noncoding RNAs (lncRNAs), and microRNAs is vital for deciphering cancer mechanisms.

Purpose of the Study:

  • To investigate the relationship among SMAD3, OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1), and miR-143-3p in cervical cancer.
  • To elucidate the functional impact of this molecular axis on cervical cancer progression.

Main Methods:

  • Quantitative real-time polymerase chain reaction and Western blot assays were used to measure mRNA and protein expression levels.
  • Cellular assays including MTT, Transwell migration, and invasion assays were performed.
  • Dual-luciferase reporter assays and transfection techniques were employed to study molecular interactions and functional effects.

Main Results:

  • SMAD3 and OIP5-AS1 expression were significantly upregulated in cervical cancer tissues and cell lines, while miR-143-3p was downregulated.
  • OIP5-AS1 overexpression promoted cervical cancer cell proliferation, migration, and invasion, whereas SMAD3 silencing inhibited these processes.
  • OIP5-AS1 was found to sponge miR-143-3p, consequently regulating SMAD3 expression.

Conclusions:

  • LncRNA OIP5-AS1 promotes cervical cancer cell migration and invasion by upregulating SMAD3 expression through sponging miR-143-3p.
  • The OIP5-AS1/miR-143-3p/SMAD3 axis represents a potential therapeutic target for cervical cancer.
Keywords:
SMAD3cervical cancerlncRNA OIP5-AS1miR-143-3pmigration

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