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Cellular changes associated with persistent hepatitis A infection in vitro.

R E Kiernan, J A Marshall, A G Coulepis

    Archives of Virology
    |January 1, 1987
    PubMed
    Summary
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    Hepatitis A virus (HAV) infection in BSC-1 cells leads to persistent antigen presence and altered cell structures. Infected cells exhibit cytoplasmic granules and unique lamellae, impacting cell division over 60 days.

    Area of Science:

    • Virology
    • Cell Biology
    • Hepatology

    Background:

    • Persistent infections can alter host cell behavior.
    • Hepatitis A virus (HAV) infects liver cells, but its long-term effects on cell ultrastructure are not fully understood.

    Purpose of the Study:

    • To investigate the long-term effects of persistent hepatitis A virus infection on BSC-1 cell division, morphology, and ultrastructure.
    • To characterize the presence and distribution of hepatitis A antigen (HAAg) in infected cells.

    Main Methods:

    • Comparison of persistently HAV-infected BSC-1 cells with uninfected controls over 60 days.
    • Immunoperoxidase staining for HAAg detection.
    • Radioimmunoassay (RIA) for quantifying HAAg levels.
    • Radioimmunofocus assay and immune electron microscopy for HAV confirmation.

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  • Thin sectioning electron microscopy for ultrastructural analysis.

    • Main Results:

    • Both infected and control cells displayed biphasic growth (exponential and stationary phases).
    • Hepatitis A antigen (HAAg) appeared in the cytoplasm during the exponential phase and was present in nearly all cells by the stationary phase.
    • HAV was confirmed in infected cells during the stationary phase.
    • Unique cytoplasmic annulate lamellae were observed in infected cells, absent in controls.

    Conclusions:

    • Persistent HAV infection influences BSC-1 cell growth dynamics and introduces distinct ultrastructural changes.
    • HAAg accumulates over time, with significant presence in the stationary phase of cell culture.