An open-source control system for in vivo fluorescence measurements from deep-brain structures

Area of Science:

• Neuroscience
• Biotechnology
• Optical Engineering

Background:

• Intracranial photometry is crucial for deep-brain signal measurement using fluorescent probes.
• Genetically encoded fluorescent reporters have enhanced the utility of this technique.

Purpose of the Study:

• To develop an open-source, cost-effective microcontroller-based system for intracranial photometry.
• To provide a flexible and customizable solution for controlling optical components and processing signals.

Main Methods:

• Designed and implemented a microcontroller-based system for intracranial photometry.
• Integrated optical components for simultaneous dual-color fluorescence measurements.
• Demonstrated proof-of-principle in freely moving mice.

Main Results:

• Achieved high-quality intracranial photometry recordings from the dorsal striatum.
• System supports simultaneous measurements in two independent color channels.
• Modular design allows integration for additional channels.

Conclusions:

• The open-source system offers increased experimental flexibility and reduced cost compared to commercial alternatives.
• Customizable software and firmware enhance adaptability for diverse research needs.
• Easy assembly and affordability make advanced photometry accessible to more researchers.