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Updated: Feb 3, 2026

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
Optimization of polysaccharides extraction from Pacific oyster (Crassostrea gigas) using subcritical water:
Adane Tilahun Getachew1, Hee Jeong Lee2, Yeon Jin Cho3
1Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Namgu, Busan 48513, Republic of Korea; School of Chemical and Bioengineering, Addis Ababa Institute of Technology, Addis Ababa University, P.O. Box 385, Addis Ababa, Ethiopia.
Abstract:
Bioactive polysaccharide was extracted from Pacific oyster Crassostrea gigas using subcritical water (SW) and the extraction process was optimize by response surface methodology (RSM). The optimum condition was found to be temperature 125.01 °C, extraction time 14.93 min, and liquid to solid ratio of 44.69:1 (ml:g). At this condition, the yield of the C. gigas polysaccharides (CGPs) was found to be 18.66%. The polysaccharide was characterized for its chemical, physical, thermal, and structural properties using HPLC, GPC, XRD, FTIR, TGA, UV-Vis spectroscopy, and NMR and the results of this characterization showed a characteristic feature of a typical polysaccharide. The CGPs was found to be a d-glucan with α-(1 → 4) configuration. The CGPs was also evaluated for its antioxidant, antihypertensive, and hypoglycemic activity and the IC50 (mg/ml) values were found to be 2.06 ± 0.33, 1.58 ± 0.03, and 2.77 ± 0.01 respectively. The current study demonstrated that SWE could be used as an effective process to extract bioactive polysaccharides from C. gigas.
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