Related Experiment Video
Updated: Feb 3, 2026

Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
Published on: July 10, 2015
Characterization of phosphate solubilizing bacteria isolated from heavy metal contaminated soils and their potential
Zedong Teng1, Wen Shao1, Keyao Zhang1
1College of Environmental Science and Engineering, Beijing Forestry University, Beijing, 100083, China; Beijing Key Lab for Source Control Technology of Water Pollution, Beijing Forestry University, Beijing, 100083, China.
Abstract:
Phosphate solubilizing bacteria (PSB) were isolated from heavy metal contaminated soils and their potentials for lead (Pb) immobilization in soil were studied in this paper. 53 PSB strains were isolated and their minimum inhibitory concentrations (MICs) for lead were determined. The results showed that strains B3, B4, B10, F2-1 and L1-5 had relatively high lead resistant capabilities with MICs ranged from 0.5 to 8 mM, and were identified as species of Leclercia adecarboxylata and Pseudomonas putida by 16S rRNA. The most efficient PSB strains could solubilize 200 mg L-1 of P, and phosphate solubilizing capacity of those PSBs was related to the concentrations of organic acids, acid phosphatase activity and pH. Furthermore, the growth pattern of L1-5 strain with the presence of Pb(NO3)2 was observed and a mechanism of biomineralization of lead ions in bacteria biomass was determined by FT-IR and XRD analyses. Results showed that high concentration of lead can inhibit the growth of PSBs, and L1-5 isolate can transform lead ions into lead hydroxyapatite and pyromorphite. The PSBs, which possessing the properties of immobilizing lead through phosphate solubilization and biomineralization could be exploited for bioremediation of Pb polluted soils in future.
Related Concept Videos
The Soil Ecosystem
Metal-Ligand Bonds
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
The Roles of Bacteria and Fungi in Plant Nutrition
Bonding in Metals
Alkali Metals
Table 1: Properties of the alkali metals
Contaminants and Errors
Another key consideration is determining the appropriate number of samples required to...

