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Updated: Feb 3, 2026

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy
Published on: August 13, 2019
Using vibrational molecular spectroscopy with chemometrics as an analytical method to investigate association of
Ningning Xu1, Jianxin Liu2, Peiqiang Yu3
1Department of Animal and Poultry Science, College of Agricultural and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada; Institute of Dairy Science, MoE Key Laboratory of Molecular Animal Nutrition, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
Abstract:
Corn starch played a critical role in maintaining energy supply for high milk yield. The objectives of this research were to disclose the starch and carbohydrate-related biopolymers degradation in three newly developed corn lines (LM10, LM01 and LD999) during rumen incubation, and detect relationships between molecular structures and starch degradation. Attenuated Total Reflectance Fourier-transform Vibrational Molecular Spectroscopy (ATR-Ft/VMS) was applied to reveal molecular structure conformations that were associated with major nutrients macro-biopolymers. Line LM01 was greater (P < 0.01) in peak heights and areas of carbohydrate (CHO) related spectral than LM10 and LD999 (P < 0.01). Line LM01 had greater rumen degradable dry matter and starch than LM10 and LD999 (P < 0.05). During 48 h rumen incubation, absorbance intensities of CHO peak 1 and peak 3 decreased linearly (P < 0.01), but absorbance intensities of CHO peak 2 increased, non-structural CHO related spectral absorbance intensities decreased linearly (P < 0.01). Correlation analysis showed that CHO associated spectral were positively correlated to ruminal dry matter and starch degradability (P < 0.10). The results inferred that the molecular spectral features of newly developed corn lines played a more important role in determining starch degradability.
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