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Updated: Feb 3, 2026

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
Published on: May 10, 2024
CRISPR-Cas9/Cas12a biotechnology and application in bacteria
Ruilian Yao1, Di Liu2, Xiao Jia1
1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
CRISPR-Cas technologies, including CRISPR-Cas9 and CRISPR-Cas12a, offer powerful tools for genome editing, gene regulation, and nucleic acid detection. These advancements are revolutionizing synthetic biology and metabolic engineering in bacteria.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR-Cas systems are prokaryotic immune mechanisms.
- CRISPR-Cas9 and CRISPR-Cas12a are widely utilized for genome engineering.
- Recent discoveries include anti-CRISPR proteins and novel Cas variants.
Purpose of the Study:
- To review CRISPR-Cas technologies, focusing on CRISPR-Cas9 and CRISPR-Cas12a.
- To explore the mechanisms, variants, and biotechnologies of CRISPR-Cas systems.
- To summarize applications in synthetic biology and metabolic engineering.
Main Methods:
- Literature review of CRISPR-Cas systems and their applications.
- Discussion of biological mechanisms, including immune defense and anti-CRISPR systems.
- Highlighting CRISPR-Cas biotechnologies like genome editing, gene regulation, and base editing.
Main Results:
- CRISPR-Cas systems function as adaptive immune mechanisms in prokaryotes.
- Novel Cas variants (e.g., xCas9, Cas13) offer unique functionalities.
- CRISPR-Cas enables precise genome editing, gene regulation, base editing, and nucleic acid detection.
Conclusions:
- CRISPR-Cas technologies have broad applications in synthetic biology and metabolic engineering.
- The versatility of CRISPR-Cas systems continues to expand with new variants and applications.
- This review provides an overview of current CRISPR-Cas capabilities and future directions.
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