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Related Experiment Video

Updated: Feb 3, 2026

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Published on: October 30, 2014

A Quantitative Model for BicD2/Cargo Interactions.

Crystal R Noell¹, Kyle M Loftus¹, Heying Cui¹

¹Department of Chemistry , State University of New York at Binghamton , Binghamton , New York 13902 , United States.

|October 23, 2018

Summary

This summary is machine-generated.

Dynein adaptor protein BicD2 shifts cargo selection during the cell cycle, preferentially binding the nucleus in G2 phase. A quantitative model reveals RanGTP as a negative regulator, suggesting another regulator is yet to be identified.

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Last Updated: Feb 3, 2026

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

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Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

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Published on: August 28, 2019

Area of Science:

Cell Biology
Molecular Mechanisms
Biophysics

Background:

Dynein adaptor proteins like Bicaudal D2 (BicD2) are crucial for intracellular transport, linking cargoes to the dynein motor.
Human BicD2 exhibits cell cycle-dependent cargo switching, transporting vesicles in G1/S phase and the nucleus in G2 phase.

Purpose of the Study:

To elucidate the molecular mechanisms governing BicD2's cell cycle-dependent cargo switching.
To develop a quantitative model of BicD2/cargo interactions.

Main Methods:

Quantitative modeling integrating affinities, oligomeric states, and reactant concentrations.
Analysis of BicD2 interactions with Rab6^GTP and Nup358.

Main Results:

BicD2 and cargo predominantly form 2:2 complexes.
BicD2 exhibits higher affinity for Nup358 (nucleus) than Rab6^GTP (vesicles).
RanGTP acts as a negative regulator, reducing Nup358/BicD2 affinity by tenfold, potentially preventing off-phase nuclear transport.

Conclusions:

The quantitative model predicts BicD2 recruitment to the nucleus via Nup358 in G2 phase.
An additional, yet unidentified, negative regulator is likely involved.
Understanding these transport dynamics is vital for brain development, cell cycle control, and synaptic function.