Reply to the Comment on "Michael Addition Based Chemodosimeter for Serum Creatinine Detection Using (...

A very simple chemodosimeter has been developed for creatinine biomolecules based on Michael addition reaction by using ( E)-3-(Pyren-2-yl)-1-(3,4,5-trimethoxyphenyl)prop-2- en-1-one Chalcone. The photophysical properties of the chalcone were thoroughly analyzed using UV-vis and emission techniques. The chalcone has exhibited two absorption maxima at 297 and 407 nm which are due n-π* and π-π* transactions, respectively. This property was further confirmed by repeating UV-vis absorbance studies of the chalcones in different solvents having different polarity. The PTP chalcone has originally exhibited ICT mechanism and it is arrested while creatinine is added. However, a ratiometric response is observed due to the creatinine induced ICT mechanism and it is also clearly supported with DFT studies. In our original work, we did DFT studies for only one isomer of the creatinine. Currently, we have extended our DFT studies for another isomer also. The relative quantum yield of the PTP chalcone was calculated in sensing and standard conditions as 0.85 and 0.45, respectively.