Influence of mouthwashes on extended exhaled nitric oxide (F_ENO) analysis

Fractional exhaled nitric oxide (F_ENO) is used to assess eosinophilic inflammation of the airways. F_ENO values are influenced by the expiratory flow rate and orally produced NO. We measured F_ENO at four different expiratory flow levels after two different mouthwashes: tap water and carbonated water. Further, we compared the alveolar NO concentration (C_ANO), maximum airway NO flux (J'_awNO) and airway NO diffusion (D_awNO) after these two mouthwashes. F_ENO was measured in 30 volunteers (healthy or asthmatic) with a chemiluminescence NO-analyser at flow rates of 30, 50, 100 and 300 mL/s. A mouthwash was performed before the measurement at every flow rate. The carbonated water mouthwash significantly reduced F_ENO compared to the tap water mouthwash at all expiratory flows: 50 mL/s (p < .001), 30 mL/s (p = .001), 100 mL/s (p < .001) and 300 mL/s (p = .004). J'_awNO was also significantly reduced (p = .017), however, there were no significant differences in C_ANO and D_awNO. In conclusion, a carbonated water mouthwash can significantly reduce oropharyngeal NO compared to a tap water mouthwash at expiratory flows of 30-300 mL/s without affecting the C_ANO and D_awNO. Therefore, mouthwashes need to be taken into account when comparing F_ENO results.