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Condensins02:15

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Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
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Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
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Small Molecule Condensin Inhibitors.

Hang Zhao1, Zoya M Petrushenko1, John K Walker2,3

  • 1Department of Chemistry and Biochemistry , University of Oklahoma , 101 Stephenson Parkway , Norman , Oklahoma 73019 , United States.

ACS Infectious Diseases
|October 23, 2018
PubMed
Summary

Researchers discovered small molecules that inhibit the bacterial condensin MukBEF, targeting essential chromosome folding processes. This work identifies potential therapeutic strategies and specific binding sites on MukB for drug development.

Keywords:
HTSMukBcondensinsdrug resistanceinhibitionnovobiocin

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Drug Discovery

Background:

  • Condensins are crucial for chromosome organization and are implicated in disease.
  • Targeting bacterial condensins like E. coli's MukBEF offers therapeutic potential.

Purpose of the Study:

  • To discover small molecule inhibitors of the Escherichia coli condensin MukBEF.
  • To identify druggable binding sites on the MukB protein.

Main Methods:

  • Pilot screening of small molecule libraries.
  • Computer-assisted molecular docking.
  • Mutational analysis and analog inspection for binding site validation.

Main Results:

  • Five compounds inhibited the MukBEF pathway; two directly affected MukB.
  • Michellamine B and NSC260594 were identified as direct MukB inhibitors.
  • Plausible binding sites in MukB's hinge and head domains were identified and validated.

Conclusions:

  • A strategy for discovering condensin inhibitors was established.
  • Specific druggable binding sites on MukB were identified.
  • Two novel small molecule inhibitors of bacterial condensins were described.