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Updated: Feb 3, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Selection of a DNA Aptamer against Zearalenone and Docking Analysis for Highly Sensitive Rapid Visual Detection with
Yuanyuan Zhang1, Taofeng Lu1, Yue Wang1
1Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute , Chinese Academy of Agricultural Sciences , Harbin 150069 , China.
Abstract:
Contamination of feed with zearalenone (ZEN) presents a significant risk to animal health. Here, a visible, rapid, and cost-effective aptamer-based method is described for the detection of ZEN. After 8 rounds of SELEX (systematic evolution of ligands by exponential enrichment) with an affinity-based monitor and counter-screening process, the ssDNA aptamer Z100 was obtained, which had high affinity (dissociation constant = 15.2 ± 3.4 nM) and good specificity. Docking analysis of Z100 indicated that noncovalent bonds (π-π interactions, hydrogen bonds, and hydrophobic interactions) helped ZEN to anchor in the binding sites. Finally, a label-free detection method based on gold nanoparticles and Z100 at 0.25 μM was developed for ZEN determination. Excellent linearity was achieved, and the lowest detection limit was 12.5 nM. This rapid and simple method for ZEN analysis has high sensitivity and can be applied for on-site detection of ZEN in animal feeds.
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