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Human Optic Nerve: An Enhanced Proteomic Expression Profile.

Gayathree Karthikkeyan1, Yashwanth Subbannayya1, Mohd Altaf Najar1

  • 11 Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University) , Mangalore, India .

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|October 23, 2018
PubMed
Summary
This summary is machine-generated.

This study presents the largest proteomic profile of the human optic nerve, identifying 5,682 proteins, including 3,222 novel ones. This research establishes a baseline for understanding optic nerve function and disease.

Keywords:
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Area of Science:

  • Neuroscience
  • Proteomics
  • Ophthalmology

Background:

  • The optic nerve and retina are crucial for visual processing, representing new frontiers in post-genomic research.
  • Previous studies have gaps in understanding the optic nerve's biochemical composition and molecular functions.
  • Proteomics offers advanced tools to explore the molecular landscape of the optic nerve.

Purpose of the Study:

  • To elucidate the human optic nerve proteomic profile using high-resolution mass spectrometry.
  • To identify novel proteins and establish a baseline proteome for the human optic nerve.
  • To provide insights into the molecular composition and potential functions of optic nerve proteins.

Main Methods:

  • High-resolution mass spectrometry (Orbitrap Fusion Tribrid) was employed.
  • Proteomic data was analyzed using SEQUEST HT and MASCOT algorithms against the NCBI Human RefSeq 75 database.
  • Label-free quantification based on spectral abundance was performed.

Main Results:

  • Identification of approximately 35,000 peptides, corresponding to 5,682 proteins in human optic nerve samples.
  • 3,222 proteins were reported for the first time in the human optic nerve proteome.
  • Abundant proteins included neuronal structural proteins (myelin basic protein, glial fibrillary acidic protein, proteolipid protein 1), neurotransmitter receptors, and postsynaptic density proteins.

Conclusions:

  • This study provides one of the most comprehensive proteomic profiles of the human optic nerve to date.
  • The identified proteome serves as a foundational resource for future research into optic nerve function and pathology.
  • Understanding the protein dynamics under normal conditions is essential for investigating disease mechanisms.