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Updated: Feb 3, 2026

Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood
Published on: April 13, 2012
Cytokines in umbilical cord blood-derived cellular product: a mechanistic insight into bone repair
Mukta S Sane1, Neha Misra1, Omid Mohammad Mousa1,2
1Department of Research & Development, Burst Biologics, Boise, ID 83705, USA.
Aim:
Umbilical cord blood (UCB) finds frequent applications in regenerative medicine. We evaluated the role of cytokines present in a uniquely processed, UCB-derived cellular allograft product (UCBp).
Materials & Methods:
Luminex multiplex assay and standard cell biology methods were employed.
Results:
Study with allografts from 33 donors identified 44 quantifiable cytokines in the UCBp derived conditioned media (CM). The UCBp-CM elevated proliferation and migration rates of mesenchymal stem cells (MSCs) and bone marrow stromal cells. Moreover, UCBp-CM induced secretion of VEGF-A and osteoprotegerin, which promoted angiogenesis of endothelial cells and positively influenced the osteogenic differentiation of MSCs, respectively.
Conclusion:
Cytokines in UCBp stimulate cellular processes important for bone regeneration, making UCBp an excellent candidate for potential applications in orthopedic procedures like bone non-union and spinal fusion.
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