Nonlinear reactions of limbic structure electrical activity in response to rhythmical photostimulation in guinea pigs

Photostimulation of the visual analyzer with a periodic signal is widely used in research and clinical practice, as well as in brain-computer interface technologies. In most studies of rhythmic photostimulation in structures of visual system at all its levels, the nonlinear nature of the response reactions is noted. However, the mechanism of formation of the induced electrophysiological reactions remains unclear. In addition, there is no literature data on the nature of response reactions of "non-visual" brain structures. The goal of the present study was to investigate the peculiarities and dynamics of the electrophysiological response of the limbic system to rhythmic photostimulation and analize the dynamics of harmonic components in the response spectra. We investigated the electrical activity of the guinea pig limbic system in response to photostimulation with a 10 Hz sinusoidal signal. Local field potentials were recorded simultaneously from the hippocampus, entorhinal cortex, medial septum and amygdala. Similar to the visual system structures, we have shown that response reactions in the limbic system had a pronounced nonlinear character, consisting in the presence of the stimulation frequency harmonics in the local field potential spectra. The correlation analysis of the dynamics of the harmonics' amplitudes did not reveal reliable relationships between them. The dynamics of the phase difference between the stimulus and individual harmonics varied in time, following different logic. Based on the results of the present work, we propose that the harmonics reveal independent processes having a different functional purpose and the nervous system operates with these harmonics independently.