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Downregulation of long noncoding RNA PVT1 attenuates paclitaxel resistance in glioma cells

Tiejun Song1,1, Lei Yan2,1, Kerui Cai2

  • 1Department of Clinical Laboratory, The Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang, China.

Cancer Biomarkers : Section a of Disease Markers
|October 24, 2018
PubMed
Abstract

Insights

Drug resistance is a problem in cancer treatment. Inhibiting long non-coding RNA PVT1 (lncRNA PVT1) in glioma cells can overcome paclitaxel resistance, increasing cell death and reducing proliferation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Drug resistance is a significant challenge in clinical cancer therapy.
  • Aberrant expression of long non-coding RNAs (lncRNAs) is implicated in various cancers, including glioma.
  • The role of specific lncRNAs, such as PVT1, in mediating drug resistance in glioma requires further investigation.

Purpose of the Study:

  • To investigate the function of the long non-coding RNA PVT1 (lncRNA PVT1) in paclitaxel-resistant human glioma cells.
  • To determine if targeting lncRNA PVT1 can overcome drug resistance in glioma treatment.

Main Methods:

  • Paclitaxel-resistant glioma cells (SHG-44 RE) were established and compared to parent cells (SHG-44).
  • Microarray analysis identified aberrantly expressed lncRNAs, with qRT-PCR validating lncRNA PVT1 overexpression in resistant cells.
  • Cell viability and apoptosis were assessed using MTT assays and flow cytometry after manipulating lncRNA PVT1 expression.

Main Results:

  • Long non-coding RNA PVT1 (lncRNA PVT1) was found to be significantly overexpressed in paclitaxel-resistant glioma cells (SHG-44 RE) compared to their sensitive counterparts (SHG-44).
  • Down-regulation of lncRNA PVT1 in SHG-44 RE cells led to decreased cell viability and increased apoptosis following paclitaxel treatment.
  • These findings indicate that inhibiting lncRNA PVT1 enhances the sensitivity of human glioma cells to paclitaxel.

Conclusions:

  • Down-regulation of lncRNA PVT1 can enhance chemosensitivity to paclitaxel, induce apoptosis, and inhibit proliferation in glioma cells.
  • Targeting lncRNA PVT1 presents a potential strategy to overcome paclitaxel resistance in clinical cancer medicine.
  • Further research into lncRNA PVT1's role could lead to improved therapeutic approaches for glioma patients experiencing drug resistance.
Keywords:
GliomaPVT1lncRNApaclitaxel resistance

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