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Updated: Feb 3, 2026

Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
Significance Testing and Multivariate Analysis of Datasets from Surface Plasmon Resonance and Surface Acoustic Wave
Mihaela Puiu1, Lucian-Gabriel Zamfir2,3, Valentin Buiculescu4
1R&D Center LaborQ, University of Bucharest, 4-12 Regina Elisabeta Blvd., Bucharest 030018, Romania. elenamihaela.puiu@g.unibuc.ro.
Abstract:
In this study, we performed uni- and multivariate data analysis on the extended binding curves of several affinity pairs: immobilized acetylcholinesterase (AChE)/bioconjugates of aflatoxin B₁(AFB₁) and immobilized anti-AFB₁ monoclonal antibody/AFB₁-protein carriers. The binding curves were recorded on three mass sensitive cells operating in batch configurations: one commercial surface plasmon resonance (SPR) sensor and two custom-made Love wave surface-acoustic wave (LW-SAW) sensors. We obtained 3D plots depicting the time-evolution of the sensor response as a function of analyte concentration using real-time SPR binding sensograms. These "calibration" surfaces exploited the transient periods of the extended kinetic curves, prior to equilibrium, creating a "fingerprint" for each analyte, in considerably shortened time frames compared to the conventional 2D calibration plots. The custom-made SAW sensors operating in different experimental conditions allowed the detection of AFB₁-protein carrier in the nanomolar range. Subsequent statistical significance tests were performed on unpaired data sets to validate the custom-made LW-SAW sensors.
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