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Related Experiment Video

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Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force

Seungyeop Choi¹, Kwanhwi Ko², Jongwon Lim³

¹Department of Biomedical Engineering, Yonsei University, Wonju 26493, Korea. sychoi0091@gmail.com.

Sensors (Basel, Switzerland)

|October 24, 2018

Summary

Cellular dielectrophoretic (DEP) behaviors were characterized using modulated frequencies, revealing nonlinear responses and distinct cross-over frequencies in living cells. This provides a new method for analyzing cell electrodynamics.

Keywords:

cross-over frequency dielectrophoresis force spectroscopy microfluidic device non-linear cellular DEP Behaviors

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Area of Science:

Biophysics
Cellular Electrodynamics
Dielectrophoresis

Background:

Characterizing cellular dielectrophoretic (DEP) behavior under alternating current (AC) electric fields is crucial for dielectrophoresis applications.
Previous studies typically used monotonic frequency sweeps, potentially missing complex cellular responses.

Purpose of the Study:

To develop a method for simultaneously tracing the DEP behaviors of numerous cells at the single-cell level.
To investigate nonlinear cellular electrodynamic responses to modulated DEP forces.
To characterize frequency-dependent cellular DEP behaviors beyond monotonic sweeps.

Main Methods:

Utilized dielectrophoretic tweezers-based force spectroscopy for robust, high-throughput cell tracing.
Applied modulated DEP forces with successive increases and decreases in electric field frequency.
Simultaneously monitored the behavior of over 150 individual cells within a single experimental environment.

Main Results:

Successfully characterized nonlinear DEP cellular behaviors in living cells.
Demonstrated the generation of different cross-over frequencies by modulating the DEP force.
Observed that living cells exhibit non-linear di-polarized responses dependent on frequency modulation direction.

Conclusions:

Living cells display nonlinear dielectrophoretic responses influenced by the direction of applied frequency modulation.
The developed platform offers a simple and reliable method for measuring cellular cross-over frequencies and their nonlinear properties.
This work advances the understanding of cellular electrodynamics and provides a valuable tool for cell analysis.