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Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles

Petra Grznárová Prokšová1,2, Jan Lipov3, Jaroslav Zelenka4

  • 1Department of Biochemistry and Microbiology, University of Chemistry and Technology, 166 28 Prague, Czech Republic. petra.grznarova@natur.cuni.cz.

Viruses
|October 24, 2018
PubMed

Insights

This study reveals how Mason-Pfizer monkey virus (M-PMV) envelope glycoprotein (Env) travels to the plasma membrane. Env is trafficked through endosomes, where immature M-PMV particles anchor, preventing recycling and enabling transport to the cell surface.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • The envelope glycoprotein (Env) is essential for retroviral entry into host cells.
  • Mason-Pfizer monkey virus (M-PMV) Env mediates the transport of immature viral particles to the plasma membrane (PM).

Purpose of the Study:

  • To elucidate the intracellular trafficking pathway of M-PMV Env and immature viral particles.
  • To identify the endosomal compartments involved in M-PMV Env transport.

Main Methods:

  • Utilized labeled RabGTPases as endosomal markers.
  • Analyzed the transport of recombinant fluorescently labeled M-PMV Gag and Env proteins.

Main Results:

  • M-PMV Env was primarily localized in Rab7a- and Rab9a-positive endosomes.
  • A proposed model suggests Env transport from the trans-Golgi network (TGN) to Rab7a-endosomes, then to Rab9a-endosomes, and back to the TGN.
  • Immature M-PMV particles anchor to Env-containing Rab9a vesicles, preventing Env recycling and facilitating transport to the PM.

Conclusions:

  • The study proposes a novel mechanism for M-PMV intracellular trafficking.
  • Rab9a-positive endosomes play a critical role in anchoring immature M-PMV particles and preventing Env recycling.
Keywords:
Mason-Pfizer monkey virusendosomesenvelopeintracellular traffickingtransportvirus-like particles

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