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Target-activatable gold nanoparticle-based aptasensing for protein biomarkers using stimuli-responsive aggregation.

Guoliang Liu1, Da-Qian Feng1, Zhiyi Li1

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|October 24, 2018
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This study presents a novel light-scattering aptasensor for detecting prostate specific antigen (PSA). The developed biosensor offers ultrasensitive and selective detection of PSA, showing great potential for clinical diagnostics.

Keywords:
Activatable aptasensorGold nanoparticlesLight-scatteringProstate-specific antigen (PSA)Stimuli-responsive aggregation

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Prostate specific antigen (PSA) is a key biomarker for prostate cancer detection.
  • Existing methods for PSA detection often lack the required sensitivity or selectivity.
  • Development of novel biosensors is crucial for early and accurate diagnosis.

Purpose of the Study:

  • To develop an ultrasensitive and selective light-scattering aptasensor for prostate specific antigen (PSA) detection.
  • To utilize bifunctional DNA-decorated gold nanoparticles (Au NPs) for target-responsive assembly.
  • To enhance PSA detection capabilities for potential clinical applications.

Main Methods:

  • Fabrication of a light-scattering aptasensor using bifunctional DNA-modified gold nanoparticles (Au NPs).
  • Employing target stimuli-responsive assembly for Au NPs aggregation.
  • Utilizing poly(dA) and aptamer domains for modulated DNA spacing and antigen binding.
  • Monitoring light-scattering intensity changes upon aptamer-PSA complex formation.

Main Results:

  • The aptasensor demonstrated ultrasensitive detection of PSA with a linear range from 0.01 ng/mL to 20 ng/mL.
  • A low detection limit of 2 pg/mL for PSA was achieved.
  • The sensor exhibited high selectivity and was successfully applied to detect PSA in serum samples.
  • The light-scattering intensity correlated directly with increasing PSA concentrations.

Conclusions:

  • The developed bifunctional DNA-decorated Au NP aptasensor provides a sensitive and selective platform for PSA detection.
  • The target-responsive assembly mechanism effectively enhances the light-scattering signal.
  • This facilely fabricated, thiol-free aptasensor shows significant promise for clinical diagnostics of prostate cancer.