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Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
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Bilinear Staphylococcus aureus detection based on suspension immunoassay.

Yaqi Shan1, Chunxiang Xu1, Mingliang Wang1

  • 1State Key Laboratory of Bioelectronics, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China.

Talanta
|October 24, 2018
PubMed
Summary

A novel bilinear suspension immunoassay biosensor offers sensitive and specific detection of Staphylococcus aureus. This method significantly reduces false positives, providing accurate quantification in milk and water samples.

Keywords:
Bilinear detectionSandwich immunoassaySimilar slopeStaphylococcus aureus

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microbiology

Background:

  • Staphylococcus aureus poses a significant threat to public health, necessitating rapid and accurate detection methods.
  • Conventional culture-based methods for bacterial detection are time-consuming and labor-intensive.
  • Development of sensitive and selective biosensors is crucial for early diagnosis and control of S. aureus infections.

Purpose of the Study:

  • To develop a novel bilinear suspension immunoassay biosensor for the specific detection of Staphylococcus aureus.
  • To achieve sensitive and selective quantification of S. aureus in various sample matrices.
  • To evaluate the performance of the developed biosensor compared to traditional methods.

Main Methods:

  • Construction of a dual-color sandwich immunosensor using monoclonal antibodies immobilized on fluorescent microspheres (PSA-R6G) as capture probes.
  • Utilizing a fluorescein isothiocyanate (FITC)-labelled antibody as a reporter probe for dual-labeling.
  • Employing multiparameter flow cytometry for the analysis of dual-labelled bacteria.
  • Establishing regression equations for quantitative analysis: IFITC = 1.56lgC + 4.50 and IR6G = 1.54lgC + 2.78.

Main Results:

  • The developed biosensor demonstrated high sensitivity and selectivity for Staphylococcus aureus detection.
  • Similar slopes in regression equations (1.56 and 1.54) indicated a significant reduction in false positives.
  • Successful quantification of S. aureus in milk and water samples was achieved.
  • The suspension immunoassay showed excellent specificity compared to conventional culture-based methods.

Conclusions:

  • The novel bilinear suspension immunoassay biosensor provides a sensitive, selective, and rapid method for Staphylococcus aureus detection.
  • The dual-color labeling and flow cytometry analysis effectively minimize false positives, enhancing detection reliability.
  • This biosensor holds promise for routine diagnostics and food/water safety monitoring.