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Updated: Feb 3, 2026

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research
Published on: July 14, 2023
Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone
Natalia Beloglazova1, Pieterjan Lenain2, Mickael Tessier3
1Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.
Abstract:
A sensitive tool for simultaneous quantitative determination of two analytes in a single spot with the use of a bioimprinted protein is presented for the first time. BSA is chosen as a scaffold for generation of binding sites specific towards two compounds. A multiplex immunosorbent assay for screening of two cereal-born mycotoxins, deoxynivalenol and zearalenone, in wheat and maize is realized with the use of fluorescent silica coated quantum dots as labels. Water-soluble fluorescent nanostructures consist of core/shell Cd-QDs enrobed in silica shells to ensure their solubility. The mycotoxins are simultaneously detected by scanning the assay outcome at two different wavelengths, since two QD@SiO2 labelled conjugates fluorescent in different parts of the spectrum. The assay is combined with a rapid extraction technique. The limits of detection for the simultaneous determination were 100 and 700 µg kg-1 in both matrices for zearalenone and deoxynivalenol, respectively. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the obtained results.
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