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Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
Published on: December 13, 2019
Correlation between pancreatic mast cells and the low grade inflammation in adipose tissue of experimental
Ahmed A M Abdel-Hamid1, Alaa El-Din L Firgany2
1Department of Histology and Cell Biology, Faculty of Medicine, Mansoura University, Egypt.
Abstract:
The role of mast cells (MCs) in prediabetes (Pre-DM) is not clearly elucidated. In the current study rats (n = 22 each) were divided equally into; control and Pre-DM (received high fat diet, HFD) groups. Samples from pancreas as well as from visceral adipose tissue (VAT) were studied for the consequent changes. We detected a significantly increased mast cell count (MCC) in the pancreas of Pre-DM compared to that of control. Frequent degranulation of MC granules was observed in Pre-DM. VAT of the Pre-DM had significantly increased (p < 0.05) macrophages (CD68+) and mast cells (tryptase+) compared to that of the control. A significant increase (p < 0.05) in CD68 mRNA expression as well as in the level of IL-1 β, IL-6, TNF-α and TGF- β1 was detected in VAT of Pre-DM with a significant positive correlation (p < 0.05) with the MCC. All these findings may indicate a potential role of MC in the low grade inflammation of VAT in Pre-DM.
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