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Related Experiment Video

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Multiscale Investigations of Cortical Processing by Integrating Laminar Polytrodes and Optogenetics with Micro Electrocorticography in Rodents
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Does Impedance Matter When Recording Spikes With Polytrodes?

Joana P Neto1,2,3, Pedro Baião1, Gonçalo Lopes2

  • 1CENIMAT/I3N and CEMOP/Uninova, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.

Frontiers in Neuroscience
|October 24, 2018
PubMed
Summary
This summary is machine-generated.

Electrode impedance minimally impacts brain recording quality. Lowering impedance using PEDOT-PSS coating does not significantly alter extracellular spike amplitude or noise, ensuring reliable neural data and spike sorting.

Keywords:
coatingimpedancemicroelectrodesnoisespikes

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Area of Science:

  • Neuroscience
  • Electrophysiology
  • Biomedical Engineering

Background:

  • Extracellular microelectrodes are vital for measuring brain activity.
  • The impact of electrode impedance on extracellular spike amplitude and background noise remains unclear.
  • Accurate spike detection and neuronal assignment depend on extracellular recording quality.

Purpose of the Study:

  • To quantify the effect of microelectrode impedance on extracellular recording data quality.
  • To evaluate impedance reduction protocols for microelectrodes.
  • To determine if electrode impedance significantly affects spike detection and sorting.

Main Methods:

  • Utilized commercial polytrodes with 32 electrodes (177 μm²) in a dense array.
  • Compared low-impedance (∼100 kΩ) modified electrodes with high-impedance (∼1 MΩ) unmodified electrodes.
  • Employed poly (3,4-ethylenedioxythiophene)-polystyrene sulfonate (PEDOT-PSS) electrodeposition to reduce impedance.
  • Recorded in vivo using a 'chess board' pattern of coated and non-coated electrodes.
  • Assessed performance based on background noise and action potential amplitude.

Main Results:

  • The PEDOT-PSS electrodeposition protocol effectively and stably reduces microelectrode impedance up to 10-fold.
  • No significant difference in background noise or detected action potential amplitude was observed between low and high impedance electrodes when using appropriate recording systems.
  • Electrode impedance within the 0.1 to 2 MΩ range does not substantially impact data quality or spike sorting.

Conclusions:

  • Microelectrode impedance is not a critical factor for high-quality extracellular recordings and accurate spike sorting.
  • The PEDOT-PSS coating is a viable method for impedance reduction.
  • Neuroscientists can be less concerned about microelectrode impedance variations within the standard range.