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Correction to: Isolation, Purification, and Culture of Primary Murine Sensory Neurons

Sarah Katzenell1, Jorge R Cabrera1, Brian J North1

  • 1Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth University, 630E Borwell Building, One Medical Center Drive, HB 7556, Lebanon, NH, 03756, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 24, 2018
PubMed

Insights

A correction was made to the concentration of mouse laminin used in experiments. The correct concentration is 18 μg/ml, not 180 μg/ml, for optimal cell culture preparation.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Materials Science

Background:

  • Accurate reagent concentrations are critical for reproducible experimental outcomes in biological research.
  • Mouse laminin is a key extracellular matrix protein used in cell culture to promote cell adhesion and differentiation.

Purpose of the Study:

  • To correct a previously published error in the concentration of mouse laminin specified in experimental protocols.
  • To ensure the accuracy of materials and methods sections for future research reproducibility.

Main Methods:

  • Identification of an error in Chapter 15, Section 2.3 regarding reagent concentration.
  • Correction of the specified concentration of mouse laminin from 180 μg/ml to 18 μg/ml.
  • Confirmation of the preparation volume of 150 μl per coverslip remains unchanged.

Main Results:

  • The concentration of mouse laminin in Hank's Balanced Salt Solution (HBSS) has been corrected.
  • The accurate concentration for preparing 150 μl per coverslip is now clearly stated as 18 μg/ml.

Conclusions:

  • This correction ensures the precise use of mouse laminin in relevant experimental procedures.
  • Adherence to the corrected protocol will enhance the reliability and validity of research findings.

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