Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

C lambda 2 and C lambda 4 immunoglobulin light chain genes in a wild-derived inbred mouse strain.

F Mami, T J Kindt

    Journal of Immunology (Baltimore, Md. : 1950)
    |June 1, 1987
    PubMed
    Summary

    Researchers identified lambda constant (C) region genes in the SPE mouse strain. The C lambda 2S gene is expressed, while C lambda 4S has a deletion, indicating differential gene evolution and selective pressure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    STUDY OF THE ASSOCIATION BETWEEN ASTHMA, WEIGHT STATUS AND NUTRITIONAL INTAKE: RESULTS OF A TUNISIAN PILOT SURVEY.

    Georgian medical news·2025
    Same author

    A common evolutionary origin for class I molecules of the MHC and T1 a region.

    Immunology today·2014
    Same author

    Genetic Characterization of a new allele of the rabbit group b C(k) allotypes.

    Immunogenetics·2011
    Same author

    HTLV-1 cell lines differ in constitutively activated signaling pathways that can be altered by cytokine exposure.

    Virology·2002
    Same author

    Thoracoscopy as a nonpharmacotherapeutic research modification for limiting postoperative chest pain.

    Journal of investigative surgery : the official journal of the Academy of Surgical Research·2001
    Same author

    Improved typing procedure for the polymorphic single-copy RLA-DQA gene of the rabbit reveals a new allele.

    Tissue antigens·2001

    Area of Science:

    • Immunogenetics
    • Comparative genomics
    • Mouse genetics

    Background:

    • The lambda constant (C) region genes are crucial components of the immunoglobulin light chain locus.
    • Understanding gene structure and function in different mouse strains aids in studying evolutionary divergence.
    • The SPE mouse strain (Mus spretus) offers a valuable model for comparative genetic analysis.

    Purpose of the Study:

    • To isolate and characterize lambda constant region genes (C lambda 2S and C lambda 4S) from the SPE mouse strain.
    • To compare the genetic sequences and functional potential of these genes with those in the BALB/c strain.
    • To investigate evolutionary relationships and selective pressures acting on immunoglobulin lambda gene families.

    Main Methods:

    • Isolation of a genomic clone containing C lambda 2S and C lambda 4S genes from an SPE mouse genomic library.

    Related Experiment Videos

  • DNA sequencing of the isolated genes and flanking regions.
  • Comparative sequence analysis with existing data from BALB/c mouse lambda genes.

    • Main Results:

    • The C lambda 2S gene in SPE is highly similar to BALB/c, suggesting it is functional and expressed.
    • The C lambda 4S gene in SPE contains a significant deletion, rendering it non-functional, unlike its BALB/c counterpart.
    • The SPE J lambda 4 gene region appears functional, contrasting with potential non-functionality in BALB/c.
    • Sequence comparisons reveal close evolutionary relationships between C lambda 1-C lambda 4 and C lambda 2-C lambda 3 gene pairs.

    Conclusions:

    • The SPE mouse strain exhibits distinct genetic variations in its lambda constant region genes compared to BALB/c.
    • Differential sequence divergence and deletions in C lambda 4S indicate varying selective pressures on duplicated gene pairs.
    • The C lambda 2 gene family appears to have undergone stronger selective pressure than the C lambda 4 gene family.