Related Experiment Video
Updated: Feb 3, 2026

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
Ex-Vivo Signal Transduction Studies in Chronic Lymphocytic Leukemia
Karly-Rai Rogers-Broadway1, Laura I Karydis1, Rachel C Dobson1
1Cancer Sciences Unit, Southampton General Hospital, University of Southampton, Southampton, UK.
Abstract:
Microenvironmental signaling is pivotal to chronic lymphocytic leukemia (CLL) pathology; therefore understanding how to investigate this pathway by both protein and chemical methods is crucial if we are to investigate and correlate biological changes with therapeutic responses in patients. Herein, we describe the use of western blotting also referred to as immunoblotting as a method that can semiquantitatively evaluate changes in protein expression following receptor engagement; this includes B cell receptor (BCR) signaling following stimulation with anti-IgM (Blunt et al. Clin Cancer Res 23(9):2313-2324, 2017). It is important to note that immunoblotting should always be combined with other quantitative methods such as flow cytometry to confirm activation of these signaling pathways (Aguilar-Hernandez et al. Blood 127(24):3015-3025, 2016).
More Related Videos
Related Concept Videos
Signal Transduction: Overview
Typically, signal transduction involves three...
Transduction
Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies
Medical History
Endocrine Signaling
Bacterial Signaling
Yeast Signaling

