Related Experiment Video For FASAY
Updated: Feb 3, 2026

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Published on: September 20, 2016
Detection and Functional Analysis of TP53 Mutations in CLL
Sarka Pavlova1,2,3, Jana Smardova4, Nikola Tom3
1Department of Internal Medicine, Hematology and Oncology, University Hospital Brno, Brno, Czech Republic.
Abstract:
Chronic lymphocytic leukemia (CLL) represents a prototype disease in which TP53 gene defects lead to inferior prognosis. Here, we present two distinct methodologies which can be used to identify TP53 mutations in CLL patients; both protocols are primarily intended for research purposes. The functional analysis of separated alleles in yeast (FASAY) can be flexibly adapted to a variable number of samples and provides an immediate functional readout of identified mutations. Amplicon-based next-generation sequencing then allows for a high throughput and accurately detects subclonal TP53 variants (sensitivity <1% of mutated cells).
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