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Updated: Feb 3, 2026

Serial Block-Face Scanning Electron Microscopy SBF-SEM of Biological Tissue Samples
Published on: March 26, 2021
The SEM electron-mirror effect in human tooth and synthetic hydroxyapatite samples
José Reyes-Gasga1, José Antonio Rodríguez-Torres2, Nancy Vargas-Becerril1
1Instituto de Física, UNAM, Circuito de la Investigación s/n, México, D.F., Mexico.
Abstract:
The characteristics of the electron-mirror effect (EME) image depend on both the scanning electron microscope parameters and the sample's physical properties. The behavior of human tooth (dentin and enamel) and synthetic hydroxyapatite samples submitted to the EME procedure is presented in this work. Polyethylene terephthalate (PET) and epoxy resin, two good EME producers, were used for comparison. A distorted EME image was observed in the obtained dentin's surface, but enamel and synthetic hydroxyapatite surfaces did not produce the EME. After ex situ calcination treatments of the teeth at 700 and 1,200°C, the EME was observed in dentin, enamel, and synthetic hydroxyapatite, but highly deformed EME images were produced. We show that these last observations are the result of the well-known charge-edge effect. After EME analysis, the calculated dielectric constant was 8.7 for dentin and 3.8 for PET. RESEARCH HIGHLIGHTS: Electron-mirror effect (EME) was observed in dentin but not in enamel or synthetic hydroxyapatite. Highly deformed EME images are produced in all samples after calcination at above 700°C. For dentin the calculated dielectric constant was 8.7 and for PET is was 3.8.
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