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Tn1545: a conjugative shuttle transposon.

P Courvalin, C Carlier

    Molecular & General Genetics : MGG
    |February 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    The conjugative shuttle transposon Tn1545, carrying antibiotic resistance genes, can transfer between bacteria. This element

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    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Tn1545 is a 25.3 kb element from Streptococcus pneumoniae BM4200.
    • It confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM).

    Purpose of the Study:

    • To investigate the transferability and transposition properties of Tn1545.
    • To understand its behavior in different bacterial hosts, including Gram-negative bacteria.

    Main Methods:

    • Cloning Tn1545 into a recombination-deficient Escherichia coli host.
    • Assessing gene expression and transferability in E. coli.
    • Observing transposition from plasmid to chromosome and vice versa.
    • Evaluating transformation and transposition in Bacillus subtilis.

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    Main Results:

    • Tn1545 was unstable when cloned in E. coli.
    • Resistance genes were expressed but not transferable between E. coli cells.
    • Tn1545 transposed to the E. coli chromosome and subsequently to a conjugative plasmid.
    • The element could be excised cleanly from the plasmid.
    • Tn1545 successfully transformed and transposed within Bacillus subtilis.

    Conclusions:

    • Tn1545 exhibits self-transferable and transposing capabilities across different bacterial genera.
    • Its properties as a conjugative shuttle transposon may explain the spread of Gram-positive genes into Gram-negative organisms.