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Related Experiment Video

Updated: Feb 3, 2026

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Published on: May 13, 2016

Microinjection in Zebrafish for Genome Editing and Functional Studies.

Wuhong Pei¹, Shawn M Burgess²

¹Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 25, 2018

Summary

Zebrafish embryos enable rapid, in vitro development for genetic studies. Microinjection allows precise manipulation of larval cells for genome editing and functional research, enhancing biological and translational studies.

Keywords:

CRISPR Functional studies Microinjection Mutagenesis Transgenesis Zebrafish

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Area of Science:

Developmental Biology
Genetics
Zebrafish as a Model Organism

Background:

Zebrafish embryos offer rapid, in vitro development, distinct from mammalian models.
Easy access to zebrafish embryos facilitates manipulation of larval molecular and cellular composition.
Microinjection is a key technique for modifying zebrafish embryos.

Purpose of the Study:

To detail the essential steps and applications of microinjection in zebrafish.
To highlight the use of microinjection for genome editing and functional studies.
To provide experimental tips for successful microinjection.

Main Methods:

Microinjection into zebrafish embryos.
Genome editing techniques.
Functional assays in zebrafish larvae.

Main Results:

Demonstration of essential microinjection steps.
Examples of applications in genome editing.
Examples of applications in functional studies.

Conclusions:

Microinjection is a powerful technique for zebrafish research.
This method supports biological and translational studies.
Experimental tips are crucial for successful microinjection outcomes.