Adhesion of Marinobacter hydrocarbonoclasticus to Surfactant-Decorated Dodecane Droplets

We investigate the effect of interfacial properties on the adhesion of bacteria at oil/water interfaces using confocal microscopy. Surfactant-decorated dodecane droplets of diameter 20-60 μm are generated using a coflow microfluidic device, introduced into an aqueous saline suspension of Marinobacter hydrocarbonoclasticus bacteria, and imaged in 3-D over time. Using image analysis algorithms, we determine the number of bacteria adhering at oil/water interfaces over time in the presence of dioctyl sodium succinate (DOSS), a component of the dispersant used in oil-spill recovery. The adsorption of bacteria at the oil/water interface follows Langmuir first-order kinetics for all droplet sizes, with the greatest areal number density of bacteria adhered to the smallest droplets. We vary the surfactant type [DOSS, dicyclohexyl sodium sulfosuccinate, dibutyl sodium sulfosuccinate, cetyltrimethylammonium bromide, and Tween 20] and concentration and examine the effects on long-time adhesion of bacteria. For a fixed droplet size, the areal density of bacteria at the interface decreases with increasing surfactant concentration because of a reduction in oil/water interfacial tension that increases the free energy of adhesion of the bacterium.