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Updated: Feb 3, 2026
11:26
Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments
Published on: March 21, 2016
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One-step solid-oil-water emulsion for sustained bioactive ranibizumab release.
Hui Yee Chua1,2, Yuan Siang Lui2, Ramya Bhuthalingam2
1a Institute for Health Technologies, Interdisciplinary Graduate School , Nanyang Technological University , Singapore , Singapore.
Expert Opinion on Drug Delivery
|October 26, 2018
Summary
A novel solid-in-oil-in-water (SOW) emulsion method effectively sustains the release of bioactive ranibizumab, a therapeutic protein for wet age-related macular degeneration (AMD), for 15 days.
Area of Science:
- Biomaterials Science
- Drug Delivery Systems
- Ophthalmology
Background:
- Therapeutic proteins require advanced delivery systems for protection and extended action.
- Ranibizumab is a key drug for treating wet age-related macular degeneration (AMD).
- Developing sustained-release formulations is crucial for improving treatment efficacy.
Purpose of the Study:
- To develop a facile method for sustained release of bioactive ranibizumab.
- To compare water-in-oil-in-water (WOW) and solid-in-oil-in-water (SOW) emulsion techniques for ranibizumab encapsulation.
- To evaluate the bioactivity and release profile of ranibizumab from PLGA-based particles.
Main Methods:
- Exploration of two emulsion techniques: WOW and SOW.
- Evaluation of ranibizumab bioactivity using ELISA and microBCA assays.
- Assessment of in vitro drug release profiles and particle surface morphology.
Main Results:
- SOW emulsion prevented ranibizumab destabilization at the water-oil interface, unlike WOW.
- SOW particles achieved a 15-day sustained release of ranibizumab, avoiding burst release.
- Released ranibizumab retained bioactivity, inhibiting endothelial cell growth.
- Smooth surface morphology of SOW microparticles contributed to suppressed burst release.
Conclusions:
- Solid-in-oil-in-water (SOW) encapsulation is an effective strategy for modulating ranibizumab release.
- The SOW method successfully maintains the bioactivity of ranibizumab during sustained release.
- This approach offers a promising delivery system for therapeutic proteins like ranibizumab in treating wet AMD.
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