Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

2.3K
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
2.3K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.6K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.6K
Design Example: Strain Gauge Bridge or Wheatstone Bridge01:15

Design Example: Strain Gauge Bridge or Wheatstone Bridge

1.0K
The utilization of strain gauges as transducers for converting mechanical strain into electrical signals is a common practice in various engineering applications. These strain gauges are frequently integrated into Wheatstone bridge circuits to accurately measure parameters such as force or pressure. Within this context, each element within the circuit exhibits a resistance that undergoes subtle variations when subjected to mechanical strain. The primary objective is to convert minuscule...
1.0K
Wheatstone Bridge01:29

Wheatstone Bridge

1.2K
An ohmmeter is a resistance-measuring device. It works by applying a voltage to a resistor of unknown resistance and measuring the current across the resistor. The resistance value is deduced using Ohm's law. Usually, the standard configuration of an ohmmeter comprises a voltmeter or an ammeter. However, such configurations are limited in accuracy because the meters alter the voltage applied to the resistor and the current that flows through it.
Thus, for accurate resistance measurements, a...
1.2K
Bridge rectifier01:24

Bridge rectifier

1.6K
The bridge rectifier is essential in electronics for efficiently converting alternating current (AC) to direct current (DC). Comprised of four diodes configured in a bridge layout, this rectifier effectively processes both the positive and negative halves of the AC waveform, making it superior to half-wave and full-wave center-tapped rectifiers in terms of voltage regulation and output stability.
Operationally, the bridge rectifier allows current flow through two of its diodes during each...
1.6K
Cross-bridge Cycle01:26

Cross-bridge Cycle

122.7K
As muscle contracts, the overlap between the thin and thick filaments increases, decreasing the length of the sarcomere—the contractile unit of the muscle—using energy in the form of ATP. At the molecular level, this is a cyclic, multistep process that involves binding and hydrolysis of ATP, and movement of actin by myosin.
122.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trans-kingdom coupling of redox signaling to environmental cell stress responses through multiphase partitioning.

bioRxiv : the preprint server for biology·2026
Same author

Profiling Selectivity for the <i>Shigella</i> Virulence Factor OspF.

Biochemistry·2026
Same author

Repurposing bacterial secretion systems for the design of living therapeutics.

Current opinion in microbiology·2026
Same author

Differential targeting of human pyroptotic caspase-5 and caspase-4 by <i>Shigella</i> OspC2 and OspC3.

mBio·2026
Same author

Differential targeting of human pyroptotic Caspase-5 and Caspase-4 by <i>Shigella</i> OspC2 and OspC3.

bioRxiv : the preprint server for biology·2026
Same author

<i>Shigella</i> OspF blocks rapid p38-dependent priming of the NAIP-NLRC4 inflammasome.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Feb 3, 2026

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
08:58

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

12.9K

INAVA-ARNO complexes bridge mucosal barrier function with inflammatory signaling.

Phi Luong1,2, Matija Hedl3, Jie Yan3

  • 1Division of Gastroenterology, Nutrition and Hepatology, Boston Children's Hospital, Boston, United States.

Elife
|October 26, 2018
PubMed
Summary
This summary is machine-generated.

The inflammatory bowel disease gene INAVA, through its CUPID domain and ARNO protein, maintains mucosal barrier function and regulates inflammation. INAVA-CUPID coordinates epithelial cell junctions and inflammatory signaling pathways.

Keywords:
ARNOIL-1bTRAF6actin assemblybiochemistrycell biologychemical biologyepithelial barrierhumaninflammatory bowel disease

More Related Videos

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
06:43

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Published on: May 26, 2021

6.4K
Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy
12:24

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

Published on: September 29, 2016

7.4K

Related Experiment Videos

Last Updated: Feb 3, 2026

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
08:58

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

12.9K
Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
06:43

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Published on: May 26, 2021

6.4K
Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy
12:24

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

Published on: September 29, 2016

7.4K

Area of Science:

  • Cell biology
  • Immunology
  • Gastroenterology

Background:

  • Mucosal surfaces require communication between the environment and epithelial cells for homeostasis.
  • Disruption of epithelial barrier function is characteristic of mucosal diseases like inflammatory bowel disease (IBD).
  • The IBD risk gene INAVA plays a role in coordinating environmental signals and barrier integrity.

Purpose of the Study:

  • To elucidate the bifunctional role of INAVA in coordinating environmental signals and epithelial barrier function.
  • To investigate the molecular mechanisms by which INAVA regulates cell-cell junctions and inflammatory signaling.
  • To understand the interaction between INAVA, its DUF3338 domain (CUPID), and the cytohesin ARNO.

Main Methods:

  • Investigated INAVA's DUF3338 domain (CUPID) interaction with ARNO.
  • Assessed F-actin assembly at the lateral membrane and its effect on cell-cell junctions and barrier function.
  • Examined INAVA relocation upon IL-1β stimulation and its role in TRAF6-dependent polyubiquitination and inflammatory signaling.
  • Studied INAVA and ARNO function in primary human macrophages stimulated with IL-1β and NOD2 agonists.

Main Results:

  • INAVA's CUPID domain binds ARNO, promoting F-actin assembly for barrier function.
  • ARNO binding to CUPID influences F-actin dynamics independently of its guanine nucleotide-exchange factor activity.
  • Upon IL-1β stimulation, INAVA relocates to cytosolic puncta, where CUPID amplifies TRAF6-dependent inflammatory signaling.
  • ARNO binding to INAVA-CUPID negatively regulates this inflammatory response.
  • INAVA and ARNO exhibit similar functions in human macrophages.

Conclusions:

  • INAVA-CUPID possesses dual functions critical for mucosal homeostasis.
  • INAVA-CUPID bridges epithelial barrier integrity with extracellular signals and inflammation.
  • ARNO directly coordinates INAVA's opposing roles in barrier function and inflammatory signaling.