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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Ribosomes01:27

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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
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Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
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Ribosomes01:27

Ribosomes

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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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Active Ribosome Profiling with RiboLace.

Massimiliano Clamer1, Toma Tebaldi2, Fabio Lauria3

  • 1Centre for Integrative Biology, University of Trento, Via Sommarive, 9 Povo, Italy; IMMAGINA Biotechnology s.r.l., Via alla cascata 56/c, Povo, Italy.

Cell Reports
|October 26, 2018
PubMed
Summary
This summary is machine-generated.

RiboLace isolates active ribosomes, overcoming limitations of standard ribosome profiling (Ribo-seq). This new method provides a global snapshot of active translation with single nucleotide resolution.

Keywords:
polysomal profilingprotein synthesisproteomepuromycinribosomeribosome profilingtranslationtranslational controltranslatome

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Area of Science:

  • Molecular Biology
  • Genomics
  • Translational Control

Background:

  • Ribosome profiling (Ribo-seq) analyzes RNA fragments protected by ribosomes to study translation.
  • Current Ribo-seq methods cannot differentiate between actively translating and inactive ribosomes.
  • Understanding active translation is crucial for deciphering gene expression mechanisms.

Purpose of the Study:

  • To develop a novel method for specifically isolating actively translating ribosomes.
  • To overcome the limitations of existing Ribo-seq techniques in distinguishing ribosome activity.
  • To enable a more accurate global analysis of translation dynamics.

Main Methods:

  • Development of RiboLace, a puromycin-based method for active ribosome isolation.
  • Utilizes an antibody-free and tag-free pull-down strategy.
  • Applicable to both in vitro and in vivo samples, requiring minimal input material.

Main Results:

  • RiboLace successfully isolates active ribosomes, distinguishing them from inactive ones.
  • The method provides high-resolution, single-nucleotide footprint data of active ribosomes.
  • Demonstrated efficiency with low input amounts and rapid application.

Conclusions:

  • RiboLace offers a significant advancement for ribosome profiling.
  • Enables precise global snapshots of active translation.
  • Facilitates deeper mechanistic insights into translational control and gene expression.