Related Experiment Video

Updated: Feb 3, 2026

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types
09:31

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

9.7K

The NCI Transcriptional Pharmacodynamics Workbench: A Tool to Examine Dynamic Expression Profiling of Therapeutic

Anne Monks1, Yingdong Zhao2, Curtis Hose1

  • 1Molecular Pharmacology Group, Frederick National Laboratory for Cancer Research sponsored by the NCI, Frederick, Maryland.

Cancer Research
|October 26, 2018
PubMed

Insights

This study details the NCI Transcriptional Pharmacodynamics Workbench (NCI TPW), a tool analyzing gene expression changes from anticancer drugs across diverse cancer cell lines to understand drug sensitivity and identify biomarkers.

Area of Science:

  • Pharmacogenomics
  • Cancer Biology
  • Bioinformatics

Background:

  • Anticancer therapy efficacy varies by tumor type.
  • Understanding drug-induced gene modulation is crucial for personalized medicine.

Purpose of the Study:

  • To identify patterns of drug-induced gene modulation across different cancer cell types.
  • To develop a resource for exploring drug sensitivity and gene expression associations.

Main Methods:

  • Gene-expression profiling of the NCI-60 cell line panel after exposure to 15 anticancer agents.
  • Integration of data into the NCI Transcriptional Pharmacodynamics Workbench (NCI TPW).
  • Analysis of gene modulation by pathway, drug target, and drug sensitivity.

Main Results:

  • Identified common transcriptional responses to anticancer agents across cell types.
  • Uncovered gene-expression changes correlated with drug sensitivity.
  • Demonstrated the utility of NCI TPW for hypothesis testing and biomarker discovery.

Conclusions:

  • The NCI TPW is a comprehensive resource for understanding tumor cell characteristics influencing sensitivity to anticancer drugs.
  • This workbench facilitates research into drug mechanisms and personalized cancer treatment strategies.

More Related Videos

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

6.5K
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

10.7K

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.5K
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.7K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Pharmacodynamics: Overview and Principles01:21

Pharmacodynamics: Overview and Principles

Pharmacodynamics is a scientific field that delves into drugs' intricate biochemical, cellular, and physiological effects on the human body. The study of pharmacodynamics helps us understand how drugs interact with the body and elicit various responses.
Most drugs' effects result from their interactions with drug receptors or targets within the body. These interactions trigger specific responses at the cellular or systemic level. Drug receptors can be found on the surfaces of cells or...
3.2K
Wood Panel Products01:18

Wood Panel Products

Wood panel products are essential materials used in construction for applications such as flooring, siding, and roofing, typically available in standard dimensions of 4 feet by 8 feet, with thicknesses varying from one-quarter of an inch to one and one-eighth inches. Among the most common types of wood panels is plywood, which is produced by gluing multiple layers of thin wood veneers under pressure. The grain of the outer veneers runs lengthwise, while the grains of the interior layers run...
381
Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
156.4K