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Updated: Feb 3, 2026

Cerebellar Regional Dissection for Molecular Analysis
Published on: December 5, 2020
Molecular dissection of nuclear paraspeckles: towards understanding the emerging world of the RNP milieu
Shinichi Nakagawa1, Tomohiro Yamazaki2, Tetsuro Hirose2
1RNA Biology Laboratory, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan nakagawas@pharm.hokudai.ac.jp.
Abstract:
Paraspeckles are nuclear bodies built on an architectural long noncoding RNA, NEAT1, and a series of studies have revealed their molecular components, fine internal structures and cellular and physiological functions. Emerging lines of evidence suggest that paraspeckle formation is elicited by phase separation of associating RNA-binding proteins containing intrinsically disordered regions, which induce ordered arrangement of paraspeckle components along NEAT1. In this review, we will summarize the history of paraspeckle research over the last couple of decades, especially focusing on the function and structure of the nuclear bodies. We also discuss the future directions of research on long noncoding RNAs that form 'RNP milieux', large and flexible phase-separated ribonucleoprotein complexes.
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