Related Experiment Video
Updated: Feb 3, 2026
09:13
Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
Published on: July 13, 2017
9.9K
Glycan Microarray-Assisted Identification of IgG Subclass Targets in Schistosomiasis
Y Y Michelle Yang1, Angela van Diepen1, Katarzyna Brzezicka2
1Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands.
Frontiers in Immunology
|October 26, 2018
Summary
Human schistosomiasis infection elicits diverse IgG subclass responses to parasite glycans. Specific IgG subclasses target unique schistosome glycan motifs, offering insights into immune evasion and potential vaccine targets.
Area of Science:
- Immunology
- Parasitology
- Glycobiology
Background:
- Schistosome infections cause chronic disease despite antibody presence.
- Effective immunity against schistosomes is not established, leading to re-infections.
- Antibody subclasses (IgG) may have varying roles in parasite clearance.
Purpose of the Study:
- Investigate IgG subclass recognition of specific schistosome glycan antigens.
- Determine if distinct IgG subclasses target unique glycan structures.
- Explore the role of IgG subclass responses in human schistosomiasis.
Main Methods:
- Sera from 41 individuals infected with *S. mansoni* were analyzed.
- Glycan microarrays with schistosome-derived and synthesized glycans were used.
- Antibody recognition by different IgG subclasses was quantified.
Main Results:
- All IgG subclasses recognized common glycan motifs like LDN, but with varied intensity.
- Specific IgG subclasses (IgG1, IgG3) targeted core N-glycan structures.
- IgG2 and IgG4 showed specificity for core α3-fucose and xylose motifs, previously linked to IgE.
Conclusions:
- Human IgG subclass responses are tailored to specific schistosome glycan structures.
- Distinct IgG subclasses may mediate different effector functions against the parasite.
- This is the first study to detail IgG subclass responses against defined helminth glycans.
Related Concept Videos
01:15Transcytosis of IgG
4.1K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
4.1K
02:34DNA Microarrays
21.1K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.1K
01:28Methods of Classification and Identification
1.2K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.2K
02:57Targeted Cancer Therapies
8.9K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
8.9K
01:33Peptide Identification Using Tandem Mass Spectrometry
8.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.4K
01:26Targets for Drug Action: Overview
10.3K
Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
10.3K