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The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
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Droplet-based microfluidics as a future tool for strain improvement in lactic acid bacteria
Jun Chen1, Mike Vestergaard1, Jing Shen1
1National Food Institute, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
FEMS Microbiology Letters
|October 26, 2018
Summary
Droplet-based microfluidics offers a powerful, miniaturized approach for screening microbial strains. This technology significantly enhances the selection of improved microbes, particularly lactic acid bacteria, for industrial applications.
Area of Science:
- Microbiology
- Biotechnology
- Bioengineering
Background:
- Industrial microbiology relies on strain development to enhance microbial performance and functionality.
- Traditional mutagenesis screening is crucial for the food industry, necessitating efficient mutant selection tools.
- High-throughput and cost-effective screening methods are vital for advancing microbial strain improvement.
Purpose of the Study:
- To review recent applications of droplet-based microfluidics in microbial strain improvement.
- To highlight the potential of microfluidics in screening lactic acid bacteria for enhanced properties.
- To discuss the advantages of miniaturized cell cultivation and phenotype interrogation.
Main Methods:
- Utilizing droplet-based microfluidics for picoliter-scale cell cultivation.
- Employing microfluidic platforms for high-throughput phenotype interrogation of microbial mutants.
- Focusing on screening methodologies applicable to lactic acid bacteria.
Main Results:
- Droplet-based microfluidics enables miniaturization for cell cultivation and phenotype analysis.
- This technology significantly facilitates the screening of microbes for improved phenotypical traits.
- Recent advancements show promise for diverse microbial applications, especially in food fermentation.
Conclusions:
- Droplet-based microfluidics is a transformative technology for microbial strain improvement.
- Its application in screening lactic acid bacteria offers substantial benefits for the food industry.
- Miniaturized, high-throughput screening is key to developing superior industrial microbial strains.
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