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Fluorescent Probes for Sugar Detection.

Danielle Bruen1, Colm Delaney1, Dermot Diamond1

  • 1Insight Centre for Data Analytics, National Centre for Sensor Research, School of Chemical Sciences , Dublin City University , Dublin 9 , Ireland.

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|October 27, 2018
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Summary
This summary is machine-generated.

New polymerizable boronic acid (BA) monomers create soft hydrogels for precise sugar sensing. These hydrogels use a cationic BA and anionic fluorophore system that detects saccharides by fluorescence recovery.

Keywords:
boronic acidfluorescenceglucosehydrogelsindirect sensingpyranine

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Area of Science:

  • Polymer Chemistry
  • Analytical Chemistry
  • Biomaterials Science

Background:

  • Boronic acids (BAs) are known for their ability to reversibly bind with diols, a characteristic utilized in saccharide sensing.
  • Developing accurate and sensitive methods for saccharide determination is crucial in various fields, including diagnostics and food science.
  • Existing saccharide sensing systems often face challenges related to stability, sensitivity, or ease of use.

Purpose of the Study:

  • To introduce a novel class of polymerizable boronic acid (BA) monomers for creating advanced saccharide-sensing materials.
  • To develop a two-component sugar-sensing system by combining cationic BAs with an anionic fluorophore, pyranine.
  • To fabricate soft hydrogels from these BA monomers and evaluate their performance in saccharide detection.

Main Methods:

  • Synthesis and characterization of novel polymerizable cationic boronic acid (BA) monomers.
  • Development of a fluorescence-based sensing assay utilizing the interaction between BAs and pyranine.
  • Investigation of the quenching and recovery of pyranine fluorescence in the presence of various saccharides in solution.
  • Polymerization of BA monomers to form cross-linked hydrogels and assessment of their saccharide-sensing capabilities.

Main Results:

  • A two-component saccharide sensing system was successfully established using cationic BAs and pyranine, where BA-pyranine complex formation quenched fluorescence.
  • Saccharide addition led to the formation of cyclic boronate esters, dissociating the complex and causing a reversible recovery of pyranine fluorescence.
  • The system demonstrated sensitivity to common monosaccharides like glucose, fructose, and galactose in solution.
  • Polymerized hydrogels exhibited similar reversible fluorescence recovery in the presence of glucose, indicating their potential as sensing materials.

Conclusions:

  • The developed polymerizable boronic acid monomers are effective building blocks for creating soft hydrogels with saccharide-sensing capabilities.
  • The fluorescence-based system offers a promising approach for accurate and sensitive determination of saccharide concentrations.
  • The hydrogel-based sensors demonstrate potential for practical applications in real-time saccharide monitoring.