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Updated: Feb 3, 2026

Isolation and In vitro Activation of Caenorhabditis elegans Sperm
Published on: January 31, 2011
Dose and time-dependent effects of sodium fluoride on sperm motility: An in vitro study
Basavalingappa Chaithra1, Halugudde Nagaraja Sarjan1, Shivabasavaiah1
11 Department of Zoology, University of Mysore, Mysore, India.
Abstract:
The present study was undertaken to investigate the toxic effects of sodium fluoride (NaF) on sperm motility and abnormality. Treatment of epididymal sperm suspensions with different doses of NaF (0.1, 1, 10, and 100 mg/ml) significantly reduced sperm motility at 15- and 30-min time intervals. Further, incubation at the same concentrations of NaF for 5-, 10-, and 15-min time intervals decreased sperm motility in a dose- and time-dependent manner. The present investigation revealed for the first time the adverse effect of lower doses of NaF (0.1 mg/ml) on sperm motility. Further, higher doses of NaF (10 and 100 mg/ml) caused a complete loss of sperm motility with a concomitant increase in sperm abnormality, which reflected the toxic effect of NaF on spermatozoa and its capability in fertilization.
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