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Isolation of a cDNA clone for human cytochrome c1 from a lambda gt11 expression library
Biochemical and Biophysical Research Communications
|May 29, 1987
Summary
Researchers identified human cytochrome c1 in a liver cDNA library using beef heart antibodies. Sequence analysis confirmed its high homology to bovine cytochrome c1, advancing our understanding of mitochondrial respiratory chain components.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- The cytochrome bc1 complex is crucial for cellular respiration.
- Understanding the human cytochrome c1 component is vital for mitochondrial research.
Purpose of the Study:
- To identify and characterize the human cytochrome c1 gene.
- To investigate the evolutionary conservation of cytochrome c1.
Main Methods:
- Screening a human liver cDNA expression library using lambda gt11.
- Epitope selection on nitrocellulose filters.
- Nucleotide sequencing and amino acid sequence analysis.
Main Results:
- Two positive recombinants were identified, indicating human cytochrome c1 presence.
- Deduced amino acid sequence showed high homology to bovine cytochrome c1.
- Confirmed the identity of human cytochrome c1 through epitope mapping.
Conclusions:
- Human cytochrome c1 shares significant sequence similarity with its bovine counterpart.
- This study provides a molecular basis for human cytochrome c1.
- The findings contribute to the understanding of mitochondrial electron transport chain evolution.