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Updated: Feb 3, 2026

Quantification of Circular RNAs Using Digital Droplet PCR
Published on: September 16, 2022
Quantitative Analysis with Droplet Digital PCR
1National Institute of Biology, Ljubljana, Slovenia. natasa.mehle@nib.si.
Abstract:
Digital PCR-based methods, such as droplet digital PCR, are one of the best tools for determination of absolute nucleic-acid copy numbers. These techniques avoid the need for reference materials with known target concentrations. Compared to real-time PCR, they provide higher accuracy of quantification at low target concentrations, and have higher resilience to inhibitors. In this Chapter, we describe the droplet digital PCR workflow for the detection and quantification of flavescence dorée phytoplasma.
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