Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Seed Structure and Early Development of the Sporophyte02:33

Seed Structure and Early Development of the Sporophyte

31.1K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
31.1K
Lung Capacity01:47

Lung Capacity

56.3K
The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures.
56.3K
Introduction to Seed Plants03:40

Introduction to Seed Plants

68.1K
Most plants are seed plants—characterized by seeds, pollen, and reduced gametophytes. Seed plants include gymnosperms and angiosperms.
68.1K
Optimal Foraging00:48

Optimal Foraging

13.8K
How animals obtain and eat their food is called foraging behavior. Foraging can include searching for plants and hunting for prey and depends on the species and environment.
13.8K
Optimization Problems01:26

Optimization Problems

71
Optimization problems often involve identifying maximum or minimum values under specific constraints. A well-known example is determining the longest horizontal pipe that can be moved around a right-angled corner, where a 3-meter-wide hallway meets a 2-meter-wide hallway. This scenario, common in architectural design and industrial transport, can be understood conceptually through geometric and trigonometric reasoning.To visualize the problem, consider the pipe as a straight line that touches...
71
Fruit Development, Structure, and Function01:58

Fruit Development, Structure, and Function

25.2K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inferior Long-Term Outcome of Fatty Liver Allografts After Orthotopic Liver Transplantation.

Annals of transplantation·2026
Same author

Prediction of Renal Graft Function 1 Year After Adult Deceased-Donor Kidney Transplantation Using Variables Available Prior to Transplantation.

Annals of transplantation·2024
Same author

Right heart failure after left ventricular assist devices: Surgical considerations.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2016
Same author

Inhibition of CaMKII Attenuates Progressing Disruption of Ca(2+) Homeostasis Upon Left Ventricular Assist Device Implantation in Human Heart Failure.

Artificial organs·2016
Same author

MR Imaging-derived Regional Pulmonary Parenchymal Perfusion and Cardiac Function for Monitoring Patients with Chronic Thromboembolic Pulmonary Hypertension before and after Pulmonary Endarterectomy.

Radiology·2016
Same author

IgM-Enriched Human Intravenous Immunoglobulin-Based Treatment of Patients With Early Donor Specific Anti-HLA Antibodies After Lung Transplantation.

Transplantation·2015

Related Experiment Video

Updated: Feb 3, 2026

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells
10:13

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

771

Identifying an optimal seeding protocol and endothelial cell substrate for biohybrid lung development.

Ulrich Zwirner1,2, Klaus Höffler3, Michael Pflaum1,2

  • 1Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.

Journal of Tissue Engineering and Regenerative Medicine
|October 27, 2018
PubMed
Summary

Optimizing endothelial cell seeding on artificial lung surfaces is crucial for biohybrid lung development. Heparin/albumin-coated poly-4-methyl-1-pentene hollow fibre membranes with specific rotational seeding and static culture protocols ensure long-term endothelialization.

Keywords:
ECMObioartificial lungbiohybrid lungendothelializationgas exchange membraneshollow fibre membranes

More Related Videos

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.
06:26

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.

Published on: September 7, 2022

5.1K
Isolation of Primary Mouse Lung Endothelial Cells
06:41

Isolation of Primary Mouse Lung Endothelial Cells

Published on: November 10, 2021

6.8K

Related Experiment Videos

Last Updated: Feb 3, 2026

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells
10:13

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

771
An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.
06:26

An Efficient Clearing Protocol for the Study of Seed Development in Tomato Solanum lycopersicum L.

Published on: September 7, 2022

5.1K
Isolation of Primary Mouse Lung Endothelial Cells
06:41

Isolation of Primary Mouse Lung Endothelial Cells

Published on: November 10, 2021

6.8K

Area of Science:

  • Biomaterials Engineering
  • Regenerative Medicine
  • Cardiovascular Engineering

Background:

  • Biohybrid lung development requires optimized haemocompatibility through endothelial cell seeding on artificial surfaces.
  • Current challenges include achieving sufficient long-term endothelialization for viable alternatives to lung transplantation.

Purpose of the Study:

  • To identify the ideal combination of polymeric hollow fibre membranes (HFMs) and seeding protocols for long-term endothelialization.
  • To evaluate the impact of different seeding strategies and inflammatory stimulation on human cord blood-derived endothelial cells (HCBECs).

Main Methods:

  • Analysis of four polymeric HFMs (polypropylene and poly-4-methyl-1-pentene) with varying pore sizes and coatings.
  • Utilized four rotational seeding protocols with HCBECs, followed by fluorescence microscopy, cell counting, and gene expression analysis.
  • Assessed cell activation, shear stress, and thrombogenic markers, as well as responsiveness to inflammatory stimuli.

Main Results:

  • Optimized long-term endothelialization was achieved using heparin/albumin-coated poly-4-methyl-1-pentene HFMs.
  • A protocol of 24 hours rotational seeding at 1 rpm followed by 120 hours static culture proved most effective.
  • Seeding methods and coatings did not negatively impact HCBEC anti-thrombogenic and anti-inflammatory status or responsiveness.

Conclusions:

  • Heparin/albumin coating and specific rotational seeding protocols are critical for successful, long-lasting endothelialization of HFMs.
  • These findings are essential for the future development and in vivo application of biohybrid lungs.
  • The study highlights the importance of optimized surface modification and cell seeding techniques in tissue engineering.