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Long noncoding RNA XIST participates in bladder cancer by downregulating p53 via binding to TET1

Bo Hu1, Guowei Shi1, Qian Li2

  • 1Department of Urology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.

Journal of Cellular Biochemistry
|October 27, 2018
PubMed

Insights

Long noncoding RNA XIST (XIST) knockdown suppresses bladder cancer cell migration and proliferation. XIST downregulates p53 by binding to TET1, revealing a novel XIST-TET1-p53 regulatory network in bladder cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in cellular regulatory networks and disease pathology.
  • The lncRNA X-inactive specific transcript (XIST) is known to influence cell migration, proliferation, and apoptosis in various cancers.

Purpose of the Study:

  • To investigate the role of XIST in bladder cancer.
  • To determine the effects of XIST on bladder cancer cell proliferation, migration, and apoptosis.
  • To elucidate the molecular mechanism underlying XIST's function in bladder cancer.

Main Methods:

  • Cell proliferation, cell cycle, and apoptosis assays were performed on bladder cancer cells.
  • Cell migration was assessed using cell migration assays.
  • RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays were used to examine the XIST-TET1 interaction and TET1's regulation of p53.
  • Western blot analysis was conducted to detect p53 expression levels following XIST manipulation.

Main Results:

  • Knockdown of XIST significantly inhibited cell migration and proliferation in vitro.
  • The study confirmed that XIST binds to TET1.
  • XIST was found to downregulate p53 expression through its interaction with TET1.

Conclusions:

  • lncRNA XIST plays a role in bladder cancer progression.
  • The XIST-TET1-p53 axis represents a potential regulatory network in bladder cancer.
  • Targeting XIST may offer a therapeutic strategy for bladder cancer.
Keywords:
10 to 11 translocation 1X-inactive specific transcriptbladder cancerlong noncoding RNAp53

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