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Integrative analysis of mRNA and microRNA expression profiles in laryngeal squamous cell carcinoma

Cumhur Gokhan Ekmekci1, Ender Coskunpinar2, Hakan Avci3

  • 1Department of Genetics, Acibadem University LABMED, Istanbul, Turkey.

Journal of Cellular Biochemistry
|October 27, 2018
PubMed

Insights

This study reveals key microRNAs (miRNAs) and messenger RNAs (mRNAs) involved in larynx cancer development. Identifying these noncoding RNAs offers new insights into cancer progression and potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Larynx cancer presents significant therapeutic challenges.
  • Recent research highlights the critical role of noncoding RNAs, particularly microRNAs (miRNAs), in cancer progression, development, and metastasis.
  • miRNAs regulate protein-coding genes, influencing cellular functions.

Purpose of the Study:

  • To analyze the expression profiles of miRNAs and messenger RNAs (mRNAs) in larynx cancer.
  • To identify specific miRNAs and mRNAs crucial for larynx cancer etiology and progression.

Main Methods:

  • Expression profiling of miRNAs and mRNAs was performed.
  • Significant differential expression was defined as over twofold changes for miRNAs and sixfold changes for mRNAs.
  • Genomic expression studies were conducted to analyze gene expression levels.

Main Results:

  • Expression levels of 43 miRNAs were increased, while 129 were decreased.
  • 97 genes were found to be upregulated, and 128 genes were downregulated.
  • hsa-miR-20a-3p and hsa-miR-1972 were identified as significant in larynx cancer etiology.

Conclusions:

  • The study identified specific differentially expressed miRNAs and mRNAs in larynx cancer.
  • These findings enhance the understanding of noncoding RNA roles in larynx cancer.
  • hsa-miR-20a-3p and hsa-miR-1972 are implicated as important factors in larynx cancer development.
Keywords:
expression profilelarynx cancermRNA (messenger RNA) expressionmicroRNAs (miRNAs)qRT-PCR

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