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Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
DgMab-6: Antihuman DGKγ Monoclonal Antibody for Immunocytochemistry
Tomoyuki Nakano1, Satoshi Ogasawara2, Toshiaki Tanaka1
11 Department of Anatomy and Cell Biology, Yamagata University Faculty of Medicine , Yamagata, Japan .
Abstract:
Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). Since both DG and PA serve as lipidic second messengers, DGK plays a pivotal role in regulating the balance of two signaling pathways mediated by DG and PA in cellular functions. Reportedly, DGKγ, one of the 10 mammalian DGK isozymes, is involved in leukemic cell differentiation, mast cell function, and membrane traffic. Transfection studies using tagged expression vectors and immunohistochemistry on rat tissues revealed that DGKγ localizes to the cytoplasm, plasma membrane, and Golgi apparatus. However, a limited number of studies reported the detailed localization of native protein of DGKγ in human tissues and cells. In this study, we developed a novel anti-DGKγ monoclonal antibody, DgMab-6, which is very useful in immunocytochemistry of human cultured cells.
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