Indoxacarb interaction alters immunotoxic and genotoxic potential of endotoxin

Area of Science:

• Toxicology
• Immunology
• Pesticide Safety

Background:

• Indoxacarb is a widely used insecticide for pest control.
• Previous toxicological data on indoxacarb exists, but its interaction with endotoxins requires further investigation.

Purpose of the Study:

• To investigate the toxicological interaction between indoxacarb and lipopolysaccharides (LPS).
• To assess the impact of indoxacarb and LPS co-administration on immune parameters and DNA damage in mice.

Main Methods:

• Male Swiss albino mice were orally administered indoxacarb (4 mg/kg/day or 2 mg/kg/day) for 90 days.
• On day 91, mice were intranasally challenged with LPS.
• Evaluated total leukocyte count, lymphocytopenia, neutrophilia, and DNA damage using comet assay.

Main Results:

• Indoxacarb (4 mg/kg) significantly decreased total leukocyte count and induced lymphocytopenia and neutrophilia.
• Both indoxacarb doses combined with LPS resulted in significant lymphocytopenia.
• While indoxacarb alone did not cause DNA damage, co-administration with LPS significantly increased DNA damage markers.

Conclusions:

• Indoxacarb at 4 mg/kg induces immune response changes after prolonged oral administration.
• Combined exposure to indoxacarb and LPS accelerates immunotoxicity and enhances endotoxin-induced DNA damage.