Itrax micro X-ray fluorescence (μXRF) for soft biological tissues

Area of Science:

• Analytical Chemistry
• Biogeochemistry
• Materials Science

Background:

• Traditional methods for determining elemental composition in soft biological tissues are time-consuming and labor-intensive.
• Existing literature lacks studies on applying Itrax micro X-ray fluorescence (μXRF) to biological samples.

Purpose of the Study:

• To adapt and evaluate the Itrax μXRF scanning instrument for elemental abundance determination in soft biological tissues.
• To establish a novel, efficient method for elemental profiling of biological samples.

Main Methods:

• Utilized Itrax μXRF, a scanning instrument with 200 μm resolution, originally designed for geological cores.
• Adapted the technique for soft biological tissue samples, involving drying, grinding into powder, and analysis.
• Standardized scanner-generated elemental counts to determine relative elemental abundance.

Main Results:

• Successfully extended the capability of Itrax μXRF to analyze soft biological tissue samples.
• Demonstrated Itrax μXRF as a simultaneous technique capable of analyzing over 30 elements at once.
• Highlighted the non-destructive nature of the method, allowing for sample re-use.

Conclusions:

• Itrax μXRF provides a significant advancement over conventional methods for elemental analysis in biological tissues.
• The technique offers minimal sample preparation, high throughput, and preserves sample integrity for further analysis.
• Acquired data holds potential for applications in environmental and biological research.